Intracellular Localization of Peptide Hydrolases in Wheat (Triticum aestivum L.) Leaves

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Intracellular Localization of Peptide Hydrolases in Wheat

Protoplasts from 8to 9-day-old wheat (Triticum aestivum L.) leaves were used to isolate organelles which were examined for their contents of peptide hydrolase enzymes and, in the case of vacuoles, other acid hydrolases. High yields of intact chloroplasts were obtained using both equilibrium density gradient centrifugation and velocity sedimentation centrifugation on sucrose-sorbitol gradients. ...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1982

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.69.3.575